Study of the association of the polymorphism of the 8q24 region and gastric adenocarcinoma
Keywords:
gastric cancer, genetic polymorphisms, rs6983267, 8q24 regionAbstract
Gastric adenocarcinoma is one of the leading causes of death in the world and in the Lara State it is the second leading cause of death from malignant neoplasms. Over the past few decades, molecular research has provided details of genetic and epigenetic abnormalities related to malignant transformation and progression in gastric cancer (GC). Recently, the association between variants of the 8q24 region and breast, prostate, stomach and colorectal cancer has been described. In this project we evaluated the association of single nucleotide polymorphism rs6983267 (G/T) of the 8q24 chromosome region with the risk of developing CG in individuals from the Center-West of the country. A case-control study was carried out using archival biopsies included in kerosene, 101 GC samples and as control 101 chronic gastritis samples (GCr). Previously there was confirmation of the histopathological diagnosis. After DNA extraction from the samples, PCRRFLP polymorphism analysis was performed using HpyCH4IV restriction enzyme. The most prevalent genotypes for GC and GCr were GG (39.6%) and GT (46.5%), respectively. In both groups of samples the G allele was predominant with 58.4% for GC and 61.9% for GCr. No significant differences in genotypic and allelic frequencies were found between both groups.
Downloads
References
Ali, Z., Deng, Y., Tang, Y., Zheng, S., Ma, N. y He, N. (2013). Epigenetic deregulations in gastric cancer. Journal of Nanosciense and Nanotechnology, 13(1): 40-51.
Ben-Porath, I., Thomson, M., Carey, V., Ge, R., Bell, G., Regev, A. y Weinberg, R. (2008). An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors. Nature Genetics, 40(5): 499-507.
Bensen J., Xu, Z., Smith, G., Mohler, J., Fontham, E. y Taylor, J. (2013). Genetic polymorphism and prostate cancer aggressiveness: A case-only study of 1,536 GWAS and candidate SNPs in African-Americans and European-Americans. Prostate, 73(1): 11–22.
Bertucci, F., Lagarde, A., Ferrari, A., Finetti, P., Charafe-Jauffret, E., Van Laere, S., y Olschwang, S. (2012). 8q24 cancer risk allele associated with major metastatic risk in inflammatory breast cancer. PLoS One, 7(5): e37943.
Cañas, M., Morán, Y., Camargo, M., Rivero, M., Bohórquez, A., Villegas, V., Chiurillo, M. (2009). Polimorfismo del codón 72 de TP53 y riesgo de cáncer gástrico: estudio caso-control en individuos de la región centroccidental de Venezuela. Revista de Investigación Clínica, 50(2): 153-61.
Cañas, M., Morán, Y., Rivero, M., Bohórquez, A., Villegas, V., Rendón, Y., Chiurillo, M. (2009). Polimorfismo genético de interleuquina-1: Asociación con cáncer gástrico en la población de alto riesgo del Centroccidente de Venezuela. Revista Médica de Chile, 137(1): 63-70.
Chiurillo, M. (2014). Role of gene polymorphisms in gastric cancer and its precursor lesions: current knowledge and perspectives in Latin American countries. World Journal of Gastroenterology, 20(16): 4503-4515.
Chiurillo, M., Moran, Y., Cañas, M., Valderrama, E., Álvarez, A. y Armanie, E. (2010). Combination of Helicobacter pylori-iceA2 and proinflammatory interleukin-1 polymorphismsis associated with the severity of histological changes in Venezuelan chronic gastritis patients. FEMS Immunology and Medical Microbiology, 59(2): 170-6.
Chiurillo, M., Moran, Y., Cañas, M., Valderrama, E. y Armanie, E. (2011). Infection with specific Helicobacter pylori-cag pathogenicity island strainsis associated with interleukin-1B gene polymorphisms in Venezuelanchronic gastritis patients.
Digestive Diseases and Sciences, 56(2): 449-56. Chiurillo, M., Moran, Y., Cañas, M., Valderrama, E., Granda, N., Sayegh, M. y Ramírez, J. (2013). Genotyping of Helicobacter pylori virulence-associated genes shows high diversity of strains infecting patients in western Venezuela. International Journal of Infectious Diseases, 17(9): e750-e756.
Ferlay, J., Shin, H., Freddie, F., David, D., Colin, C. y Maxwell, D. (2010). Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. International Journal of Cancer, 127(12): 2893–2917.
Ferrari, F. y Moura, M. (2013). Study of risk factors for gastric cancer by populational databases analysis. World Journal Gastroenterol, 19(48): 9383-9391.
Figueiredo, C., García-González, M. y Machado, J. (2013). Molecular pathogenesis of gastric cancer. Helicobacter Supplement, 1(18): 28-33.
Guo, Y., Fang, J., Liu, Y., Sheng, H., Zhang, X., Chai, H., Gao, H. (2011). Association between polymorphism rs6983267 and gastric cancer risk in Chinese population. World Journal of Gastroenterology, 17(13): 1759-1765.
Hochedlinger, K.,Yamada, Y., Beard, C. y Jaenisch, R. (2005). Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell, 121(3): 465-477.
Jang, B. y Kim, W. (2011) Molecular pathology of gastric carcinoma. Pathobiology, 78(6): 302-10.
Laurén, P. y Nevalainen, T. (1993). Epidemiology of intestinal and diffuse types of gastric carcinoma. A time trend study in Finland, with comparison between studies from high? and low risk areas. Cancer, 71(10): 2926-2933.
Laurén, P. (1965). The two histological main types of gastric carcinoma: diffuse and socalled intestinal type carcinoma. Acta Pathologica et Microbiologica Scandinavica, 64: 31-49.
Lichtenstein, P., Holm, N., Verkasalo, P., Iliadou, A., Kaprio, J., Koskenvuo, M., Hemminki, K. (2000). Environmental and heritable factors in the causation of cancer-analyses of cohorts of twins from Sweden, Denmark, and Finland. The New England Journal of Medicine, 343(2): 78-85.
Ministerio Del Poder Popular Para La Salud. República Bolivariana de Venezuela. (2012). Anuario de Mortalidad 2011. Recuperado de: http://www.mpps.gob.ve
Organización Mundial De La Salud. (2011). Cáncer. Nota descriptiva N°297. Recuperado de: http://www.who.int/ mediacentre/factsheets/fs297/es/index.html.
Pomerantz, M., Ahmadiyeh, N., Jia, L., Herman, P., Verzi, M., Doddapaneni, H., Freedman, M. (2009). The 8q24 cancer risk variant rs6983267 shows long-range interaction with MYC in colorectal cancer. Nature Genetics. 41(8): 882-884.
Robbins, C., Torres, J., Hooker, S., Bonilla, C., Hernandez, W., Candreva, A., Carpten, J. (2007). Confirmation study of prostate cancer risk variants at 8q24 in African Americans identifies a novel risk locus. Genome Research, 17(12): 1717- 1722.
Terada, N., Tsuchiya, N., Ma, Z., Shimizu, Y., Kobayashi, T., Nakamura, E., Ogawa, O. (2008). Association of genetic polymorphisms at 8q24 with the risk of prostate cancer in a Japanese population. Prostate, 68(15): 1689-1695.
Tomlinson, I., Webb, E., Carvajal-Carmona, L., Broderick, P., Kemp, Z., Spain, S., Houlston, R. (2007). A genome-wide association scan of tag SNPs identifies a susceptibility variant for colorectal cancer at 8q24.21. Nature Genetics, 39(8): 984-948.
Torres, K., Moran, Y., Valderrama, E. y Chiurillo, M. (2013). Variantes del motivo EPIYA de la proteína CagA de Helicobacter pylori en biopsias gástricas de pacientes con gastritis crónica de la región centroccidental de Venezuela. Revista de la Sociedad Venenezolana de Microbiología, 33(1): 18-23.
Wokołorczyk, D., Lubiński, J., Narod, S. y Cybulski, C. (2009). Genetic heterogeneity of 8q24 region in susceptibility to cancer. Journal of the National Cancer Institute, 101(4): 278-279.
Zanke, B., Greenwood, C., Rangrej, J., Kustra, R., Tenesa, A., arrington, S., Dunlop, M. (2007). Genome-wide association scan identifies a colorectal cancer susceptibility locus on chromosome 8q24. Nature Genetics, 40(5): 631-637.
Zhang, Y., Yi, P., Chen, W., Ming, J., Zhu, B., Li, Z., Miao, X. (2014). Association between polymorphisms within the susceptibility region 8q24 and breast cancer in a Chinese population. Tumour Biology, 35(3): 2649-2654.
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
