Evaluation and comparison of the sensitivity of the primers that amplify genes msp2 and msp5 de anaplasma marginale for the diagnosis of the bovine anaplasmosis
Keywords:
diagnóstico, primers, PCR, A. marginale, diagnosisAbstract
The bacterium A. marginale infects the erythrocytes in bovines, causing an anemic picture that affects the animal's productivity, being necessary the application of sensitive and specific diagnostic methods for the control and treatment of the disease. In this sense, molecular techniques have become an ideal tool for diagnosis, therefore, taking into account that in Venezuela the PCR technique has been developed using the primers 19A and 19B that amplify the msp5 gene of the bacterium and The recent design of the primers msp2A and msp2B, which amplify the msp2 gene, aimed to evaluate the sensitivity of both pairs of primers, as well as their potential for molecular diagnosis.
Downloads
References
Barbet, A.F.; Lundgren, A.; Yi, J.; Rurangirwa, F.R.; Palmer, G.H. (2000). Antigenic variation of Anaplasma marginale by Expression of MSP2 Mosaics. Infection and Immunity. 68(11):6133-6138.
Barry, D.N., Parker, R.J., De Vos, A.J., Dunster, P., Rodwell, B.J. (1986). A Microplate Enzime-Linked Immunoabsorbent Assay for Measuring Antibody to Anaplasma marginale in Cattle Serum. Australian Veterinary Journal. 63(3): 76-79.
Brayton, K. A., Kappmeyer, L. S., Herndon, D. R., Dark, M. J., Tibbals, D. L., Palmer, G. H. Knowles, D. P. (2005). Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins. Proceedings of the National Academy of Sciences. 102(3), 844-849.
Díaz, D.; Valera, Z.; Andrade, E.; Parra, O.; Escalona, F.; Ramírez, R. (2003). Prevalencia de Anaplasma marginale en Bovinos, Sector La Piñata, Municipio La Cañada de Urdaneta, Estado Zulia, Venezuela. Revista Científica de la Facultad de Ciencias Veterinarias-LUZ. 13(3):193-198.
Eleizalde, M.C.; Caballero, H., Reyna-Bello, A. (2007). Evaluación y Mejoramiento del Ensayo Inmunoenzimático (ELISA) Para Diagnóstico de la Anaplasmosis Bovina. Revista Científica. Facultad de Ciencias Veterinarias-LUZ. 17(4): 349-356.
Eleizalde, M.C.; Reyna-Bello, A. (2006). Diagnóstico por PCR de la Anaplasmosis Bovina Utilizando el gen msp2 de A. marginale. AsoVAC. Cumaná-Venezuela.
Fernández, D.; Eleizalde, M.C.; González-Baradat, B.; González- Marcano, E.; Perrone, T.; Mendoza, M. (2009). Trypanosoma evansi: A Comparison of PCR and Parasitological Diagnosis Test in Experimentally Infected Mice. Experimental Parasitology. 121: 1-7.
Harris, E. (1998). A Low-cost Approach to PCR. New York, USA, Oxford University Press. 304pp.
Knowles, D., Torioni de Ecahide, S., Palmer, G.H., McGuire, T.C., Stiller, D., McElwain, T.F. (1996). Antibody against an Anaplasma marginale MSP5 Epítope common to Tick and Erythrocyte Stages Identifies Persistently Infected Cattle. Journal of Clinical Microbiology. 34:2225-2230.
Kocan, K.M., De la Fuente, J., Guglielmone, J. A., Meléndez, R.D. (2003). Antigens and alternatives for control of Anaplasma marginale infection cattle. Clinical Microbiology Review. 16(4): 698-712.
Montenegro-James, S., Guillén, T., Tapang, P., Abdel-Gawad, A., Toro, M. and Ristic, M. (1990). Use of the Dot Enzime-Linked Inmunoabsorbent Assay with Isolate Anaplasma marginale Initial Bodies for Serodiagnosis of Anaplasmosis in Cattle. American Journal Veterinary Research. 51(10): 1518-1521.
OIE. Organización Internacional de Epizootia. 2008. Manual de la OIE sobre animales terrestres. Cap. 2.4.1. Anaplasmosis bovina. pp: 1-13.
Reyna-Bello, A.; Cloeckaert, A.; Vizcaíno, N.; Gonzatti, M.; Aso, P.; Dubray, G.; Zigmunt, M. (1998). Evaluation of an Enzyme-Linked Inmunoabsorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela. Clinical and Diagnostic Laboratory Inmunology. 5(2): 259-262.
Rivera, M. (1996). Hemoparasitosis Bovina. Caracas, Universidad Central de Venezuela. Ediciones Anauco. 238pp.
Tavares-Marques, L., Nunez, C., Rey- Valerion, C., Reyna-Bello, A. 2010. Serological evidence of Anaplasma spp. in small ruminants from Venezuela using recombinant MSP5 in immunoenzymatic assay. Revista Científica Facultad de Ciencias Veterinarias -LUZ. 20(5): 506- 511
Tavares-Marques, L.; Reyna-Bello, A. (2006). Estandarización de la técnica de PCR para el diagnóstico de la anaplasmosis bovina y ovina. Agronomía Tropical. (56)4: 501-502.
Torioni de Echaide, S. ; Knowles, D.; McGuire, T.; Palmer, G.; Suarez, C.; McElwain, T. (1998). Detection of Cattle Naturally Infected with Anaplasma marginale in a Region of Endemicity by Nested PCR and a Competitive Enzime Linked Immunoabsorbent Assay Using Recombinant Major Surface Protein 5. Journal of clinical Microbiology. 36(3): 777-782.
Visser, E.; McGuire, T.; Palmer, G.; Davis, W.; Shkap, V.; Pipano, E.; Knowles, Jr. (1992). The Anaplasma marginale msp5 Gene Encode a 19 Kilodalton Protein Conserved in All Recognized Anaplasma Species. Infection and immunity. 60(12): 5139- 5144.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
