Application New Methodology in The Molecular Epidemiology of Chagas's Disease

Authors

  • Giannina Recchimuzzi Central University of Venezuela (CUOV)
  • Ileana Carrillo Central University of Venezuela (CUOV)
  • Hernán Carrasco Central University of Venezuela (CUOV)

Keywords:

Trypanosoma cruzi, molecular epidemiology, disease badly of chagas

Abstract

In the present study there was achieved to establish a protocol of work by means of the application of the technology PCR - RFLP, which consists of the amplification for PCR of specific regions of the genome of T. cruzi and his later digestion with enzymes of restriction, allowing the genotipificación it completes of different isolated of T.cruzi.

Downloads

Download data is not yet available.

References

Brisse, S.; Henriksson, J.; Barnabe, C.; Douzery, E.; Berkvens, D.; Serrano, M. (2003). Evidence for genetic exchange and hybridization in Trypanosoma cruzi based on nucleotide sequences and molecular karyotype. Infect. Genet. Evol. 2: 173-183.

Di Noia, J.; Buscaglia, C.; De Marchi, C.; Almeida, I.; Frasch, A. (2002). A Trypanosoma cruzi small surface molecule provides the first immunological evidence that Chagas disease is due to a single parasite lineages. J. Exp. Med. 195: 401-413.

Downloads

Published

2024-06-13

How to Cite

Recchimuzzi, G., Carrillo, I., & Carrasco, H. (2024). Application New Methodology in The Molecular Epidemiology of Chagas’s Disease. Observador Del Conocimiento, 1(1), 93–100. Retrieved from https://revistaoc.oncti.gob.ve/index.php/odc/article/view/622

Issue

Vol. 1 No. 1 (2013): diciembre

Section

Artículos

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.